SOLVED: Explain how a Lineweaver-Burk plot is generated by indicating what x and y axes represent. Showing all steps, derive the equation applied to generate the plot from the following equation:Vmax [S] / (Km [S])Explain how Vmax and Km are calculated from the graph's intercepts.Show that Km = [S] when the initial rate is half its maximum value.An enzyme has Km = 5.4 * 10^-4 M, Vmax = 48 pM/min, [S] = 0.001 M. Calculate the Vo value.
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SOLVED: Explain how a Lineweaver-Burk plot is generated by indicating what x and y axes represent. Showing all steps, derive the equation applied to generate the plot from the
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Question 1: (20 points) Using the following data set, graph the primary plot, Lineweaver-Burk plot, and calculate Kᵤ and Vmax (rounded to the nearest thousandth) for the enzyme. Also, determine what type of inhibitor each inhibitor is and provide a Lineweaver-Burk plot that shows the enzyme and its inhibited rate. There should be one Lineweaver-Burk graph for each inhibitor.[S]Enzyme rateInhibited enzyme rate 0.2Inhibited enzyme rate 2271 0.64
Question 1: (20 points) Using the following data set, graph the primary plot, Lineweaver-Burk plot, and calculate Kᵤ and Vmax (rounded to the nearest thousandth) for the enzyme. Also, determine what type of inhibitor each inhibitor is and provide a Lineweaver-Burk plot that shows the enzyme and its inhibited rate. There should be one Lineweaver-Burk graph for each inhibitor.[S]Enzyme rateInhibited enzyme rate 0.2Inhibited enzyme rate 2271 0.64
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